Method for E-Coli determination in Fecal Sludge

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  • Nida
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Method for E-Coli determination in Fecal Sludge

Dear All,

I need a suggestion.
As I am working on characterization of fecal sludge and i need to identify pathogens and indicator microorganisms, i will be determining E-Coli and helminth eggs. Can you guys help me in identifying which technique is better to use for EColi (quantification) in FS:
1. MPN
2. MF
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  • yemioludaniels
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Re: Reply: Method for E-Coli determination in Fecal Sludge

MF determination is better because is more accurate than MPN
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  • lajipaille
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Re: Reply: Method for E-Coli determination in Fecal Sludge

I prefer MF determination because in my opinion it is more precise...
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  • IlhaSolteira
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Re: Method for E-Coli determination in Fecal Sludge

Good morning.
I prefer MPN. I use Colitag Kit because it is simple and have good results.
www.colitag.com/
Best regards.
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  • caetano
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  • Caetano Dorea is an Associate Professor at the University of Victoria where he leads the Public Health & Environmental Engineering (PH2E) Lab, Canada’s only research group primarily dedicated to water, sanitation, and hygiene (WASH). His interests and expertise are at the crossroads of environmental and public health engineering.
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Re: Method for E-Coli determination in Fecal Sludge

"six of one, half a dozen of the other"... the E. coli levels in FS will be so high that it is likely your characterisation will be looking at orders of magnitude (i.e. log levels) and I'd be surprised to see a significant difference between the methods. As long as you are following/adapting a reasonably known method, I'd base your method of choice selection on price, ease of use, availability of reagents/materials, and other practical considerations.

Caetano
University of Victoria
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  • Nida
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Re: Method for E-Coli determination in Fecal Sludge

Thank you Caetano for your reply.
I will be diluting sampling upto 10 log to determine the concentration.
Due to guidance on this forum, I am now preferring MPN as low cost option
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  • Nida
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Re: Method for E-Coli determination in Fecal Sludge

Thank you for letting me know about the Colitag kit but I am wondering if it can help in quantification as well?
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  • muench
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Re: Method for E-Coli determination in Fecal Sludge

Hi Nida,
Why would you even want to determine E. coli in fecal sludge? The only pathogenic indicator of relevance in fecal sludge are helminth eggs, I would have thought. They are a good indicator to compare different treatment methods for faecal sludge because they are so hard to destroy or remove.
(see the other threads in this sub-category for more information on helminth egg determination:
forum.susana.org/207-determining-helmint...asurement-techniques )

E. coli is more for water and wastewater samples as far as I know.

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  • caetano
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  • Caetano Dorea is an Associate Professor at the University of Victoria where he leads the Public Health & Environmental Engineering (PH2E) Lab, Canada’s only research group primarily dedicated to water, sanitation, and hygiene (WASH). His interests and expertise are at the crossroads of environmental and public health engineering.
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Re: Method for E-Coli determination in Fecal Sludge

Agreed that helminths are more resistant and useful indicator organisms (in most cases), but some regulations may still require you to test for E. coli. So, it is not all that bad to test for it, if you have the resources to do so. Besides, depending on the treatment/context you are using, it may be also useful to have a less resistant indicator organism (i.e. if you are not even managing to inactivate those, then you have a more fundamental problem).

Caetano
University of Victoria
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