Wanting a better way to test pathogen inactivation? Us too! Can you help me crowdsource a better way?
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TOPIC: Wanting a better way to test pathogen inactivation? Us too! Can you help me crowdsource a better way?

Re: Wanting a better way to test pathogen inactivation? Us too! Can you help me crowdsource a better way? 05 May 2014 16:06 #8465

  • Florian
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Jan 2014
joeturner wrote:
It seems to me to be a bit of a myth that one can monitor the temperature of a compost windrow with a single temperature probe.


I think it is still easier to do muliple temperature probes in different points of a windrow at various time intevalls, than a single helminth egg analysis. In particular if this is meant as rountine process control.
Florian Klingel
Water and Sanitation Specialist at Skat Consulting Ltd.
Last Edit: 05 May 2014 16:07 by Florian.

Re: Wanting a better way to test pathogen inactivation? Us too! Can you help me crowdsource a better way? 06 May 2014 21:20 #8495

  • joeturner
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A bit of a tangent, but has anyone heard about this paper microscope?

www.scidev.net/global/biotechnology/news...aper-microscope.html
www.foldscope.com/

My first thought when I heard about it was that it might be a good thing to use for counting Ascaris.
I don't work for anyone, I am a philosopher interested to think about how we think about WASH and sanitation. All thoughts are mine alone, I am responsible for any errors.

Previously trained and worked as a Soil Scientist and worked on projects composting sewage sludge.
The following user(s) like this post: JKMakowka

Re: Wanting a better way to test pathogen inactivation? Us too! Can you help me crowdsource a better way? 12 May 2014 18:03 #8589

  • BJimenezC
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Dear Colleagues

I agree with many of you on the importance of monitoring helminth eggs. Bacteria, nor viruses nor protozoan are indicator of them. As the current internationally used methods is based on a simple but tedious and long technique to separate first the eggs from the detritus found in wastewater, excreta or sludge , to secondly, - once the eggs have been isolated - to identified and count them through an also time consuming step demanding skilled analysis.
As the methods is complicated, I think it is better to measure all the eggs and not only Ascaris, the additional effort is not very important. Moreover, even if Ascaris is the most abundant specie, its proportion is different in different regions. For practical purpose it is not that important to measure viability, given that this is linked to the integrity of the shell which is destroyed by most of the inactivating procedures. Nevertheless to assess the efficiency of a new treatment technology measuring viability is important.
I am attaching some papers on the comparison of helminth eggs analytical techniques and as well an alternative procedure to estimate the eggs number in wastewater based on the TSS content provided a calibration curve has been previously developed.
A last comment but critical, is that even if the part of the methodology to separate the eggs from the detritus is very time consuming, most of the errors on the quantification of the eggs come from the visual identification through the microscope.
Thanks to a grant from the Melinda and Gates Foundation, my lab has already develop an algorithm to recognized and count the eggs of several species found in wastewater, sludge and excreta from different regions of the world. We are now developing ‘a friendly interphase’. The idea behind is that routine laboratories will be separating the eggs from detritus using VERY simple material and that once the eggs are separated photos can be taken and sent to a central laboratory to do the counting. This will reduce costs and increase the reliability of the process
Blanca


Moderator note for attachment 2 and 3:

Reprinted from Water Science and Technology, volume 53, issue number 7, pages 43-49, with permission from the copyright holders, IWA Publishing.
http://www.iwaponline.com/wst/05405/wst054050169.htm

Reprinted from Water Science and Technology, volume 54, issue number 5, pages 169-177, with permission from the copyright holders, IWA Publishing.
http://www.iwaponline.com/wst/05307/wst053070043.htm

[Moderator note made by Sebastian Klos]
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Last Edit: 13 Jun 2014 11:17 by secretariat.

Re: Wanting a better way to test pathogen inactivation? Us too! Can you help me crowdsource a better way? 13 May 2014 23:52 #8610

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Dear Ms. BJimenez

The work developed by your lab is very important for the rapid diagnosis of helminths, We are very interested in learning more about this work
In Bolivia we are proving new methods for producing safe human organic waste (free of pathogens Ascaris and Trichuris especially) we would like to establish a line of contact with you.

Raul Silveti
This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Fundacion Sumja Huasi
www.sumaj.org

Re: Wanting a better way to test pathogen inactivation? Us too! Can you help me crowdsource a better way? 15 Jul 2014 13:30 #9358

  • muench
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For readers following this thread on "a better way to test pathogen inactivation", I would like to draw your attention to a related post by Blanca Jimenez from Mexico. She writes about the development of software to identify and quantify helminth eggs.

You can find it here on the forum:
forum.susana.org/forum/categories/97-ena...ico-unam-mexico#9351
Dr. Elisabeth von Muench
Independent consultant
Frankfurt, Germany
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Twitter: @EvMuench
Website: www.ostella.de
Member of SuSanA (www.susana.org)
Last Edit: 15 Jul 2014 13:30 by muench.
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